Protein_Domain

Part:BBa_M36143:Design

Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-22)


Inhibitor I29 Attached to Cysteine Proteinase EP-B2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 33
    Illegal BglII site found at 702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 370
    Illegal AgeI site found at 454
    Illegal AgeI site found at 793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Precursor (Pro EP-B2) and mature EBp-B2 in order to test if inhibitor is necessary for survival of cells.

Source

DNA sequence for precursor and mature EP-B2 generated from amino acid sequence in "Computational Design of an α-Gliadin Peptidase".

References